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Diagnosis of Lyme borreliosis

Your patient is suspected to have Lyme disease. Serology was inconclusive, PCR didn’t work? Are you looking for a more reliable diagnostic method? You have found one that was tested on over 40,000 samples.

(picture: Dr. Bózsik is explaining his poster to Willy Burgdorfer, who discovered the causative agent of Lyme borreliosis)

Lyme borreliosis is one of the most frequent and problematic diseases, it may affect 10% of the population (cumulated). It is caused by a spirochete, Borrelia Burgdorferi. There is severe underreporting, and the disease is highly difficult to diagnose:

  • it may affect almost any organ of the body, there may be dermatological, rheumatological, neurological, cardiac, etc. symptoms
  • the serological diagnostic methods are not reliable, especially in the early phase of the disease
  • immune response is absent, late, waving, or weak, hence false negatives in 40-50% for ELISA or Western-Blot
  • too low concentration of borrelia in the blood: microscopy, cultivation and even PCR have 15-20% success
  • borrelia are too thin for normal light microscopic investigation, and there are many artificial products formed in blood that are similar to borrelia

DualDur reagent and method was developed based on 30 years of experience in examining spirochetes, and diagnosing Lyme disease.

It is not dependent on the immune reaction of the body, which may be absent, especially in the early stages of the disease. It doesn’t search for the genetic make-up of the bacteria.

It is pure morphology: it will show spirochetes in the blood.

However, morphological investigation of spirochetes in blood can be misleading:

  • borrelia disintegrate fast in fresh blood: there is a need to feed them
  • there are not enough spirochetes in a drop of blood: need to concentrate
  • artificial products are formed after taking the blood: need to stop them forming
  • elements of human blood may look like spirochetes: need to stop the motion of human cells

DualDur is a reagent&method that solves all the above problems.

We will see only the spirochetes, 1,000x concentrated, alive and moving.

 

Afzelii 2

This fact was confirmed by immunofluorescence and PCR. We carried out 40,000 investigations with DualDur, while carefully observing and recording clinical symptoms, and performing 110,000 serological tests to back up the diagnosis.

While there may be spirochetes in many parts of the body, e.g. the mouth, to the best knowledge of medicine today, spirochetes in blood are always human pathogenic.

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